Determination of Genetic Integrity in Long-term Micropropagated Plantlets of Allium ampeloprasum L. Using ISSR Markers
نویسندگان
چکیده
منابع مشابه
Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers
A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cult...
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Plant regeneration through rapid in vitro clonal propagation of nodal explants of Morus alba L. variety S-1 was established along with genetic stability analysis of regenerates. Axillary shoot bud proliferation was achieved on Murashige and Skoog (MS) medium in various culture regimes. Highest number of shoots (5.62 ± 0.01), with average length 4.19 ± 0.01 cm, was initially achieved with medium...
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Genus Allium L. contains very taxonomically complex sections, especially the subgenus Melanocrommyum. The systematic position of the species in each section has been revised many times over time. In the present study, the relationship between 32 ecotypes belonging to 10 different species of Allium was investigated using ISSR markers. The nine primers used produced 166 polymorphic bands (average...
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Randomly amplified polymorphic DNA (RAPD) markers were used to analyze genetic stability of the somatic embryogenesis-derived regenerants (R1-6) and mother plant in Iranian date palm (Phoenix dactylifera L.) cultivar Khanizi. Total genomic DNA extracted from in vitro fresh leaves of regenerated plants and mother plant was amplified using 10-mer oligonucleotide Fermantas primers. Four primers of...
متن کاملGenetic analysis of castor (Ricinus communis L.) using ISSR markers
Castor (Ricinus communis L.) is one of the most ancient medicinal oil crops in the world. It has been vastly distributed in different parts of Iran. In the present study, the inter simple sequence repeat (ISSR) markers were used to evaluate the molecular genetic diversity among and within 12 castor accessions collected from 7 regions of Iran. Totally, 16 ISSR primers amplified 166 loci...
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ژورنال
عنوان ژورنال: Biotechnology(Faisalabad)
سال: 2010
ISSN: 1682-296X
DOI: 10.3923/biotech.2010.218.223